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  • Description:


     

    Collection of intact viable cells without enzymatic processing.
    Trypsin is well known for its deterioration to the functions of cells. Maintenance of their activities is possible with Cosmo’s
    advanced culture ware is used for cell culture.
    Harvest cells by mere reduction of temperature.
    It completely do without trypsin when collecting cells since cell detachs by just the culture surface response. So that the
    Processing would save time 80%.
    96 multiwell format is especially suitable for cell cloning by limited dilution.

    Featured

    Made of special polymer Material
    Temperature responsive polymer is fixed to the surface of the cultureware at nano-thickness level. Cells are cultured at 37 degrees Celsius and collectable approximately 10-30min after controlling temeperature to 20-25 degrees Celsius.
    Applications
    Ideal Suit for harvesting fragile cells such as stem cells and neurons, and macrophages cells etc. Better results can be offer Collection of intact and viable single cell suspension. Stem cell culture and tissue culture . Research on extra cellular matrix.

    Instructions of usage
    Culturing cells
    First of all, pre-warm the medium to 37 degrees celsius in the incubator.
    When seeding cells, please do use the pre-warmed medium to ease attachment of cells.
    Seed cells evenly over the culture surface to prevent cells being concentrated in one area.
    The surface becomes hydrophobic above 32 degrees celsius therefore cells must be cultured above this point.
    Medium change can be carried out as if using normal cultureware without polymer. Please use pre-warmed medium for change.
    When cells are difficult to attach, please use medium that contains FCS.
    Attachment time of the cells may vary depending on the type of the cell.
    Detachment of cells
    The surface of the cultureware becomes hydrophilic under 32 degrees celsius. Cells are easier to detach under this temperature.
    When collecting cells, lower the temperature down to approximately 20 degrees celsius and wait about 30 minutes. Depending on the type of the cells cultured, they detach automatically.
    Same results can be achieved by leaving the cultureware in the refrigerator set at 4 degrees celsius for 10 minutes.
    The cells that did not come off automatically can be detached by agitating them by pipet with medium.
    Some cells may not detach completely. The results may vary by the type of the cells.
    Detachment of cells are easier when the temperature is lowered before cells become confluent.
    The product is intended for one time use only. Please do not re-use the product.
    The product is only for laboratory use.

    Cat.No.NameDescriptionPackingUnitPriceQuantityTotal
    1102-03535mm DishTemperature responsive surface10dishes/Sleeves,50dishes/BoxBox$500.00
     
    1102-09696well MicroplateTemperature responsive surfaceIndividual,10plates/BoxBox$300.00
     
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